LUYOR-3415用于觀察小鼠黑色素瘤轉移

2022年10月重慶醫(yī)科大學在《Biological Research》發(fā)表《Melanoma stem cells promote metastasis via exosomal miR-1268a inactivation of autophagy》文獻，文獻中實驗采用了LUYOR-3415RG雙波長熒光蛋白激發(fā)光源和LUV-50A熒光觀察眼鏡觀察紅色熒光蛋白RFP在小鼠肺部腫瘤細胞的上的表達。

To study the effect of miR-1268a overexpression on OL cells metastasis in vivo, we generated OL cells stably overexpressing miR-1268a via lentiviral infection (Fig. 4J). 5 次 10^{5} OE-NC-OL and OE-miR-1268a-OL cells were injected into NOD/SCID mice via the tail vein, respectively (Sect. “Materials and methods”). All mice were sacrificed at day 35 after tumor cell injection. Since OE-NC-OL and OE-miR-1268a-OL cells were RFP-labeled, macroscopic metastatic foci formed at the lung could be visualize under external fluorescence imaging using LUYOR-3415 Dual Fluorescent Protein Excitation Light Source and LUV-50A Glasses. As shown in Fig. 4K, injected OE-miR-1268a-OL cells produced significantly more macroscopic lung foci than the OE-NC-OL cells (n?=?6). Subsequently, H&E analysis was performed on the largest cross-sectional lung sections of the two groups of mice. Statistical analysis showed that there were significantly more microscopic metastatic foci in the lungs of mice treated with OE-miR-1268a-OL cells (The black arrows show the formed metastases) (Fig. 4L). These observations show that miR-1268a can enhance metastatic colonization efficiency of OL cells that are oligometastatic. So far, we have demonstrated that miR-1268a, transferred from OL-SCs-derived exosomes to OL cells, can effectively augment the clonogenic colonization capability of OL cells to give arise to more extensive macroscopic metastases.

文獻地址：https://biolres.biomedcentral.com/articles/10.1186/s40659-022-00397-z 

上海峰志儀器有限公司現(xiàn)貨供應美國路陽生產的LUYOR-3415RG手持式雙波長熒光蛋白激發(fā)光源，能夠觀察綠色熒光蛋白和1紅色熒光蛋白在小鼠、斑馬魚、線蟲等模式動物上的表達。

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重慶醫(yī)科大學介紹：

重慶醫(yī)科大學創(chuàng)建于1956年，由上海醫(yī)學院（現(xiàn)復旦大學上海醫(yī)學院）分遷來渝組建，原名重慶醫(yī)學院，1985年更名為重慶醫(yī)科大學。學校是國務院學位委員會批準的首批具有博士和碩士學位授予權的單位，是教育部批準的首批來華留學示范基地。學校2015年成為重慶市人民政府、衛(wèi)生健康委員會（原衛(wèi)生計生委）和教育部共建高校，2016年成為“高等學校學科創(chuàng)新引智計劃”（“111計劃”）首批立項的地方高校之一。學校由位于渝中區(qū)的袁家崗校區(qū)和位于重慶大學城的縉云校區(qū)組成，校園總面積2650畝，其中縉云校區(qū)占地2100畝。校園環(huán)境優(yōu)美，風景如畫。

重慶醫(yī)科大學現(xiàn)有全日制在校學生26810人，其中博士、碩士研究生7124人，本科生18824人，?？粕?58人（對口支援少數(shù)民族項目），留學生604人。學校具有招收博士后研究人員，港澳臺學生和外國留學生資格。學?，F(xiàn)有8個一級學科博士學位授權點、13個一級學科碩士學位授權點，3個博士專業(yè)學位授權點、11個碩士專業(yè)學位授權點；4個重點學科，8個學科進入ESI全球前1%（2022年1月科睿唯安數(shù)據(jù)），4個重慶市學科，16個重慶市“十四五”市級重點學科。

文章轉自：https://www.luyoruv.com/news/news1/673.html