經(jīng)濟(jì)實(shí)用的熒光檢測(cè)GFP手電筒

經(jīng)濟(jì)實(shí)用的熒光檢測(cè)GFP手電筒

產(chǎn)品名稱：熒光蛋白檢測(cè)雙波長(zhǎng)GFP手電筒

目錄編號(hào)：DFP-GC

使用這款 DFP-GC LED GFP手電筒（便攜式激發(fā)光源），可以輕松檢測(cè)小鼠組織中的熒光蛋白表達(dá)。一個(gè)很好的便攜式DFP-1 GFP手電筒能夠替代昂貴的熒光立體顯微鏡。

實(shí)驗(yàn)設(shè)計(jì)和結(jié)果總結(jié)

應(yīng)用：轉(zhuǎn)基因小鼠篩選

起始材料：小鼠胚胎或解剖大腦

協(xié)議概述

1) 將鼠標(biāo)或解剖的大腦放入開(kāi)放容器中 2) 戴上特殊眼鏡（根據(jù)熒光團(tuán)選擇） 3) 打開(kāi)手電筒（開(kāi)關(guān)允許在熒光團(tuán)之間進(jìn)行選擇） 4) 關(guān)閉房間燈 5)觀察

：對(duì)于高表達(dá)熒光團(tuán)，不需要解剖也可用于新生小鼠，它可以代替基因分型。我們使用綠色和紅色貼紙點(diǎn)來(lái)標(biāo)記眼鏡和開(kāi)關(guān)，以便于使用。

總結(jié)

這是檢查您的子宮內(nèi)電穿孔大腦是否表達(dá) RFP 或 GFP 的好方法。價(jià)格僅為熒光立體顯微鏡的一小部分。解剖后的大腦可以浸沒(méi)在液體中，準(zhǔn)備切片。電穿孔的點(diǎn)更亮并且明顯受限。它看起來(lái)很小，因?yàn)槟鷽](méi)有放大鏡，我認(rèn)為是因?yàn)槟荒芸吹捷^亮的（中間）區(qū)域。您可以在工作臺(tái)上快速檢查并繼續(xù)實(shí)驗(yàn)。此外，我們使用DFP-1 GFP手電筒在 P10 之前對(duì)表達(dá) YFP 的小鼠進(jìn)行基因分型。它可以裝在箱子里運(yùn)送到動(dòng)物設(shè)施。

功能總結(jié)

您可以使用一個(gè)手電筒輕松篩選兩種不同顏色的熒光團(tuán)。

補(bǔ)充筆記

美國(guó)nightsea提供良好的技術(shù)支持，可幫助您在購(gòu)買前決定過(guò)濾器/熒光觀察眼鏡

概括：

好處：以顯微鏡價(jià)格的一小部分進(jìn)行快速篩選?？梢酝ㄟ^(guò)EMS訂購(gòu)

缺點(diǎn)：過(guò)濾器不是很好。例如，在整個(gè)大腦中 GFP 高表達(dá)的小鼠中，您無(wú)法從子宮內(nèi)電穿孔的載體中檢測(cè)到 RFP 的表達(dá)。

總結(jié)：我們推薦這款GFP手電筒在神經(jīng)科學(xué)實(shí)驗(yàn)室快速篩查熒光。

以上內(nèi)容為機(jī)器翻譯，僅供參考，以下面英文內(nèi)容為準(zhǔn)！

推薦閱讀：便攜式熒光蛋白激發(fā)光源LUYOR-3415RG 

上海峰志儀器有限公司代理銷售美國(guó)nightsea、美國(guó)路陽(yáng)便攜式熒光蛋白手電筒，有單波長(zhǎng)、雙波長(zhǎng)以及體視顯微鏡熒光適配器。具體優(yōu)惠價(jià)格請(qǐng)按照網(wǎng)頁(yè)底部的聯(lián)系方式電話或.聯(lián)系。

熒光手電筒觀察GFP的表達(dá)效果

熒光手電筒觀察紅色熒光蛋白的表達(dá)效果

Cheap and Practical Fluorescence Detector GFP Flashlight

Product Name:DFP Flashlight

Catalog Number:DFP-GC

We can easily detect fluorescent protein expression in mouse tissue with this LED flashlight. A great portable alternative to an expensive fluorescent stereomicroscope.

Experimental Design and Results Summary

Application

Transgenic mice screening

Starting Material

Mouse embryos or dissected brain

Protocol Overview

1) Place the mouse or dissected brain in an open container 2) Put on the special glasses (select depending on the fluorophore) 3) Turn on the flashlight (the switch allows to select between fluorophores) 4) Turn off the room lights 5) Observe

Tips

For high expression fluorophores no dissection is needed also used on newborn mice it can replace genotyping. We used green and red sticker dots to label glasses and switches for easy use.

Results Summary

This is a great way to check if your in-utero electroporated brains express RFP or GFP. At just a fraction of the price of a fluorescence stereo-microscope. The dissected brain can be submerged in liquid, ready for slicing. The electroporated spot is much brighter and clearly confined. it looks small because you don't have an amplification lens and I think because you only see the brighter (middle) region. You can check very fast over your bench and continue the experiment. In addition we use the flashlight to genotype YFP-expressing mice before P10. It can be transported in its case to the animal facility.

Features Summary

You can easily screen for two different colored fluorophores with one flashlight.

Additional Notes

Good tech support at nightsea.com to help you decide on filters/glasses beforr buying

Summary

The Good：Fast screening at a fraction of the price for a microscope. Can be ordered through EMS

The Bad：The filters are not very good. For example, in a mouse with high expression of GFP in all the brain you can't detect expression of RFP from a vector electroporated in-utero.

The Bottom Line：We would recommend this flashlight for fast screening of fluorescence in a neuroscience laboratory